CHANGE Xylazine  Respiratory rate:- 80-230breaths/min Cardboard • e. Adopting emergency plans. A • Institutions should employ people trained in HAEMATOLOGICAL DATA OF COMMON LABORATORY ANIMALS DISPOSAL execution stage or which involve the production of either micro-organisms or  Respiratory rate:- 85 breaths/min CPCSEA Standard Operating Procedures (SOP) for IAEC 1-7 2.   Ketamine has been used in combination with various other anesthetic drugs but it is most • Use of animals in such studies requires a special Conference on HarmonizationICH Organization for 160 - 410 500 - 1300 200 - 500 250 - 850 250 - 656 300 -700 200 - 900 109 - 597 The relative humidity should • WHAT IS CPCSEA female18-35gms care. While using an anaesthetics various side effects such as excessive CPCSEA Guidelines on Control and Supervision on Experiments on Animals.  Blood volume:- 1.5-2.5ml58.5ml/kg material 0.1-0.9  Detailed information about instrument model no. Parmar. material upto200 • GOAL 19 Slide20: The method should in all cases meet the following requirements : (a) Death, without causing anxiety, pain or distress with minimum time lag phase. 3Provide for animal housing. © 2014 authorSTREAM. A 160 - 180 ailments in animals - liters - weaning - maintenance - record keeping. It must also be ensured that the anaesthesia is given for the full duration of experiment and at no stage the animal is conscious to perceive pain during the experiment. • Sheltered or outdoor housing • Diagnostic clinical laboratory may be made that live in water aquatic air aenar land terrestrial - wild animals and 43slide 44: ENVIROMENT Need of CPCSEA To ensure that lab animals are well maintained and experiments are conducted according to ethical norms. involved in such projects. material • Visit to animal house and laboratory where the 651.4 n mol/l required clothing masks afrons gloves other route care staff maintain a high • CPCSEA is a statutory committee/body which is Provision of suitable safe 11 Slide12: WALLS & CEILINGS Walls should be free of cracks, unsealed utility penetrations, or imperfect junctions with doors, ceilings, floors and corners. • Ministry of environment forest and climatic change • The Institutional Biosafety Committee shall be the point for interaction within animals have been determined. animals should be away from human habitations and not exposed emergency power should be available in Carbon Monoxide Deputy secretory • Should have continous access to fresh potable A 18slide 19: Animal procurement 48 available. A nominee of CPCSEAimportant link between CPCSEA 100 i. 3.5-7.2 1500 IP • Recommanded floor 109.6 148.3 187 258 387 451.5 pathogenic agents/large scale used. Guidelines on the Regulation of Scientific Experiments on Animals 9-21 3. Bilirubin A • Introduction - Definition of plants and animals - types of animals – animals without hour. 15slide 16: GLP 41slide 42: FUNCTIONAL AREAS after completion of study through periodic • Action against establishments in case of violation of any Hamster/Gerbil/ r RECORD KEEPING contamination proper operation. 25 i/v NR Follow up medical check ups their weight size and size of the cage • Selection and appointment of nominees in the Guinea pig 350 ANIMAL CARE AND TECHNICAL PERSONNEL Animal care programs require technical and husbandry support. 24-35 IM 22-33 IM 11-22 IM 5-15 Synthetic the Prevention of cruelty of animal act 1960. 0.3-1 stores washing office staff machine room quarantine and 30 knockout stock. 1 • Knockout animals should be maintained in clean 3000 • Unnecessary animal killing should not be practiced. • Provision should be made for animals with specialized locomotor pattern material and excessive moisture. IP in Haryana state in 2018. 46-178 25 Transgenic animals are used to study:- including pathological test should be done periodically at annually for scientific workers Xylazine Copy, Automatically changes to Flash or non-Flash embed, WordPress Embed laboratory animal science and medicine. CPCSEA guidelines Committee for the purpose of control and supervision of experiments on animals. 87 IP WHY SHOULD WE SUFFER THROUGH EXPERIMENTATION • Example climatic condition species of animal 33slide 34: PHYSICAL RELATIONSHIP OF ANIMAL 40-60 IP  Should includes all interaction among individual of a groups 2000 - 4000 40 with procedures specified for the purpose by the Dog 15 8.0 - Rabbit Cat Dog primate 2.5-3.8 187.0 human primates Overdose route minimum time lag phase. to ensure normal growth in immature Metal secretary MoEFCC. dust used for bedding. ONE The main functions of CPCSEA are:- c Power and lighting:- System should be safe and 1. Upto80  Life span:- 1-3 years 1 or 2 toxic chemicals or other substance  Weight :- male300-500 gms SODIUM (d) Location should be separate from animal rooms and free from environmental contaminants. • REFERENCE 200+10 Maximum number i Head of the institution or his nominee • c. Training of personnel on bio safety. imperfect junctions with doors ceiling floors and corners.  Allergens:- Dander Urinary Protein 57slide 58: prolonged anaesthesia: terminal procedures only Neutrophlis 10 - 40 9 - 34 10 - 42 28 - 44 20 - 75 35 - 75 60 - 70 5 - 88 500 9-31.5 maneka gandhi. 6. SANITATION IP IP 32.5x21cm 11 8 7 6 standard of personal cleanliness. upto400 material good animal care. • An quarantine will reduce/minimize the chance of animals. KTEAMINE in genetic engineering research and production activities. treatment and control. if handling poisonous animals like venomous snakes - first aid. experimentation with hazardous agents. Weight in grams sq.ft sq.meter inches 38slide 39: Annexure – 3D Transport 25 eight members as follows:- • IAEC WE DON’T WEAR MAKE-UP OR PERFUME. provide half yearly reports on the ongoing projects to RCGM regarding the observance of the AIV • Sanitation practice should be monitored by the visual To promote human care of animals used in biomedical and behavioral research. mg/dl ANIMAL PROCUREMENT All animals must be acquired lawfully as per the CPCSEA guidelines. dichlorvos air embolisim 25 35-53 metal Metal A scientist from outside the institute 5.0  Birth weight:- 40-90 gms cage Anawal Example for calculating the number of rats to be kept per cage based on floor AIM/IP Mouse Rat Hamster Guinea upto25 34slide 35: Annexure – 3A At no stage of experiment the animal is conscious to perceive the pain. emotional effect on the operator. corridors where such work should be performed as per guidelines. FOR ANY SPECIES OF ANIMALS CPCSEA GUIDELINES FOR POULTRY & BIRDS FACILITY - 2020; View More. NR 75+10 0.25-0.74 Weight floor area Height A animal safety. Metal Mouse Rat Hamster G.pig Rabbit Cat Dog • Laboratory animals are very sensitive to their living conditions so them. e.g. DIRECTOR NIAWNational Institute of Animal Welfare 5.4-7.5 th surgical support area preparation area operating area area for intensive  Mode of transportdepends on distance seasonal and ANNEXURE – 4 ANIMAL PROCUREMENT IBSC will provide half yearly area recommended per animal according to their weight size and size of the 4 4.0 24 small animalsmice rat rabbit etc.. - 1 week Monkey • Inhalation methods _ Maximum Curariform drugs nicotinic sulphate magnesium m mol/l 10 -33 i/v 8 animal experimentation or breeding of animals for this • Recommended space requirement for animal rooms for different • Diets should be free from heavy metalslead arsenic cadmium lead Mastomy/Cotton Rhesus 55slide 56: ANAESTHESIA AND EUTHANASIA Used in NR Surface should 8-40 species are given in Annexure-3 • The animal rooms should occupy about 50-60 of total experimental work should be made and may be subjected to inspection on Material program for incoming animals  Accessible to all animals sufficient ventilation and prevent build up waste dry grass crushed corn cobs etc.. NR CPCSEA GUIDELINES Committee for the Purpose of Control and Supervision on Experiments on Animals 1. in inches In order to view it, please 148.3 Sq. Mouse Rat Hamster G. Ppig Rabbit Cat Dog URETHANE 1000 IP 1000 i/p 1500 IP 1500 i/p 1000 i/p 750 IV • Approval of Animal House Facilities on the basis of recommended space is in the foot note below both formal and on the job CPCSEA Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) Statutory body formed by the Act of the Indian Parliament under the Prevention of Cruelty to Animals Act, 1960 Formed in 1964 Revived in 1998, under the committed chairpersonship of Meneka Gandhi Head quarters at Chennai Includes various guidelines and sub committees 54slide 55: Mouse Rat Hamster G. pig Rabbit Cat Dog Primate intravenous or intramuscular injection. AIV 25 a PHYSICAL METHODS NR Carbon Dioxide plus  Provide adequate spacefreedom of movement area recommended for animal according to their weight size and size of the cage • It was revived in 1998 under the chairpersonship of All anesthetic agents to reduce the paining sensation. • The transgenic and knockout animals should be material  S.GOWRI SHANKAR • The current committee was constituted on 30 • Caging and housing system 2.6-4.1 0.2-0.8 6-7.5 APRIL  Heart rate:- 330-480 beats/min 50 Usually IM is not recommended in Mice due height of box   Animal Cm. protection require separation of animal facilities from personnel pathogen free or transgenic ganotobiotic or 42slide 43: PHYSICAL FACILITIES 5slide 6: CPCSEA facilities to be used must be reviewed by both the reports on the ongoing projects to RCGM regarding the observance of the safety guidelines by comma or enter. PROCEDURSSOPs/GUIDELINES non-laboratory bred animals - diurnal and nocturnal animals suitable and relevant areassuch as office conference room and other laboratories. 32slide 33: PHYSICAL PLANT 80 - 100 route CPCSEA GUIDELINES FOR LABORATORY ANIMAL FACILITY (committee for the purpose of control and supervision on experiments on animal) 2. years unless adequate justification is provided. Animal House staff record – both technical and non – technical Health record of staff/ animals All SOPs relevant to the animals Breeding, stock, purchase and sales records Records of experiments conducted with the number of animals used Death Record Clinical record of sick animals. 40+5 IP 20+1 IM 5+1.5 • Unexpected deaths and sign of illness distress or d Exterior windows:- not recommended for small animals. AIV iii. Euthanasiaderived from Greek word known as Upto100 0.25-0.6 Compatibility with the purpose of study and minimum piece of paper tissue paper cotton Synthetic  Good Laboratory Practices GLP for animal facilities is 7. mg/dl Cardboard  Name of manufacturer of reagents:- of hygiene cpcsea guidelines for laboratory animal facility Cpcea disposable wear such as gloves ,masks, head covers, coats, shoe covers. PCV 39 - 49 36 - 48 36 - 55 37 - 48 36 - 48 30 - 45 37 - 55 26 - 48 psychologic and nutritional stabilization before their use. Metal 2.5-4.0 1.2-5.8 HUSK BED The … windows can be provided. Overdose route supply to a limited area. APRIL h Storage area:- separate area for food bedding cages refrigerated storage cold 49slide 50: ASSESSING THE EFFECTIVENESS OF the requirements under the guidelines. weight /size and behavioural activity established colony. tuberculosis the group should be kept intact and A area per animal institution for implementation of the guidelines. d Noise control:- Noise free environment. Hb g/dl 10.2 - 16.6 11 - 18 10 - 16 11 - 15 10 – 15.5 8 - 15 12 - 18 8.8 -16.5 The animal should remain under veterinary care till it completely recovers from anaesthesia. material Local anaesthetics are used in minor and rapid procedure to block the nerve WE DON’T TAKE DRUGS 387.0 Committee for the Purpose of Control and Supervision on Experiments on Animals WHY ARE THERE LAWS ABOUT ANIMALS?  Cages should be cleaned each time before animals are placed in Most web browsers block content or generate a “mixed content” warning when users access web pages via HTTPS that contain embedded content loaded via HTTP.  Provide adequate ventilation To prevent users from facing this, Use HTTPS option. Floor area Height b corridor:- wide enough kept clean. and is the responsibility of a veterinarian or a Institutional Biosafety Committee IBSC Last Updated On 13 Jan 2021. AIP monitored recorded reported and archived. reports of inspections conducted by CPCSEA. 68. Vice chairperson CPCSEA • Adequate veterinary care must be provided 50-135 Both are essential in an animal facility. accountability. upto 30 12.0 - n mol/l protocol only approve by the IAEC. uncontaminated drinking water. • Animals rooms - animals chambers/cages - sizes of animal chambers general dimensions 2 Numeric tags are not allowed. suffering from uncontrollable paining sensation QUARANTINE, STABILIZATION AND SEPARATION Quarantine is the separation of newly received animals from those already in the facility until the health and possibly the microbial status of the newly received animals have been determined. of • Certificate of animal handling and welfare from any recognized institution 59slide 60: Species Mouse rat Hamste g Walls ceiling:- free of cracks unsealed utility penetration and free of • d. Instituting health monitoring program for laboratory personnel Complete medical check • Health status should indicate conventional • No permission to eat drink smoke or apply cosmetics in 2000 -4000 consideration Such as procedures and the ATROPINE: Dose 0.02 – 0.05 mg/kg for all species by s/c or i/m or i/v routes used be procured from CPCSEA registered breeders or Method should meet the following requirements:- Albumin 6slide 7: • The Board consists of 28 members serve for 10slide 11: CPCSEA MEMBERS  Easy access to food and water In the event of a decision to sacrifice an animal on termination of an experiment or otherwise, an approved method of euthanasia should be adopted. AIVIP behavior of animals by the animal house staff. In order to view it, please • These are the animals whose germ line foreign genes have Supervision of Experiments on Animals. of Animals weight kg floor area/anmalft² Height 1.5-2.8 Synthetic AIM/IV 0.1-2 • Preferred method for waste disposal is incineration. vi. RBCx10⁶ /mm³ 7 – 12.5 7 -10 6 - 10 4.5 - 7 4 - 7 5 - 10 5.5 – 8.5 3.56- 6.96 Because STANDARD OPERATING PROCEDURES (SOPs) / Guidelines A SOP should contain the following items : 14 Slide15: DISPOSAL The transgenic and knockout animals should be first euthanized and then disposed off as prescribed elsewhere in the guidelines. • Incinerators should be compliance with all central